poly i c hmw lyovec Search Results


poly i c  (InvivoGen)
95
InvivoGen poly i c
CD8 + T cell response can be enhanced by adding Poly (I:C) and GM CSF/IL-4 to DRibble-pulsed PBMCs. Poly (I:C) and CD40L were added with UbiLT3 pp65 DRibbles (25ug/ml) to rested PBMCs. The mean ± SEM of 3 separate experiments are shown. (A) represents the percentage of IFN-γ producing CD8 + T cell and (B) represents the CD4 + T cell response. (C) PBMCs were cultured with or without GM-CSF and IL-4 for 12 hours, then Poly (I:C) and CD40L were added to PBMCs with UbiLT3 pp65 DRibbles (25ug/ml). The mean ± SEM of 3 separate experiments are shown.
Poly I C, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+i+c+hmw+lyovec/Poly(I%3AC)+(HMW)+LyoVec/pmc04021424-63-15-18
Average 95 stars, based on 1 article reviews
poly i c - by Bioz Stars, 2026-11
95/100 stars
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CD8 + T cell response can be enhanced by adding Poly (I:C) and GM CSF/IL-4 to DRibble-pulsed PBMCs. Poly (I:C) and CD40L were added with UbiLT3 pp65 DRibbles (25ug/ml) to rested PBMCs. The mean ± SEM of 3 separate experiments are shown. (A) represents the percentage of IFN-γ producing CD8 + T cell and (B) represents the CD4 + T cell response. (C) PBMCs were cultured with or without GM-CSF and IL-4 for 12 hours, then Poly (I:C) and CD40L were added to PBMCs with UbiLT3 pp65 DRibbles (25ug/ml). The mean ± SEM of 3 separate experiments are shown.

Journal: Journal of Translational Medicine

Article Title: Cross-presentation of viral antigens in dribbles leads to efficient activation of virus-specific human memory t cells

doi: 10.1186/1479-5876-12-100

Figure Lengend Snippet: CD8 + T cell response can be enhanced by adding Poly (I:C) and GM CSF/IL-4 to DRibble-pulsed PBMCs. Poly (I:C) and CD40L were added with UbiLT3 pp65 DRibbles (25ug/ml) to rested PBMCs. The mean ± SEM of 3 separate experiments are shown. (A) represents the percentage of IFN-γ producing CD8 + T cell and (B) represents the CD4 + T cell response. (C) PBMCs were cultured with or without GM-CSF and IL-4 for 12 hours, then Poly (I:C) and CD40L were added to PBMCs with UbiLT3 pp65 DRibbles (25ug/ml). The mean ± SEM of 3 separate experiments are shown.

Article Snippet: Some factors were added at the same time with antigens including Lipopolysaccharide (100 ng/ml, immunechem), Poly(I:C) (25 ug/ml, invivogen, tlrl-piclv), M52-CpG (30 ug/ml, Sigma), R848 (10 ug/ml, invivogen, tlrl-r848), TLR2 ligand (300 ng/ml, invivogen, tlrl-pms), CD40 ligand (1 ug/ml, NIH repository, D759060X), IL-1α(300 ng/ml, NIH repository, Zlva0903), TNF-α (1000 U/ml, Cetus, 94608), and IFN - γ (100 ng/ml, peprotech, 30002).

Techniques: Cell Culture